A general insert label for peptide display on chimeric filamentous bacteriophages
نویسندگان
چکیده
منابع مشابه
Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection
The display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DNA fragment exist in one phage particle. Development of phage display antib...
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IG OF Ff BACTERIOPHAGES ............................... 101 ORGANIZATION OF GENES AND DNA............................... 101 rho-Dependent Terminator in Region [A] ............................... 101 Morphogenetic Signal............................... 102 Minus-Strand Synthesis............................... 102 Plus-StrandSynthesis.0 ............. ** ............. 103 CONCLUSION.4 ......... 10...
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Practical applications of bacteriophages in medicine and biotechnology induce a great need for technologies of phage purification. None of the popular methods offer solutions for separation of a phage from another similar phage. We used affinity chromatography combined with competitive phage display (i) to purify T4 bacteriophage from bacterial debris and (ii) to separate T4 from other contamin...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2012
ISSN: 0003-2697
DOI: 10.1016/j.ab.2011.08.050